This Article Full Text (OnlineFirst PDF) All Versions of this Article: 0269881107081521v1 22/5/536    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Robbins, M. J Articles by Maycox, P. R Search for Related Content PubMed PubMed Citation Articles by Robbins, M. J Articles by Maycox, P. R Social Bookmarking                         What's this?

Differential expression of IEG mRNA in rat brain following acute treatment with clozapine or haloperidol: a semi-quantitative RT-PCR study

¹ Department of Biology, Psychiatry Centre of Excellence for Drug Discovery, GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK.
² Statistical Sciences Europe, GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK.

^* To whom correspondence should be addressed.

	Abstract

Anti-psychotic drugs have been shown to modulate immediate early gene (IEG) expression in rat brain regions that are associated with schizophrenia, which may be directly linked to their immediate therapeutic benefit. In this study, we analysed the expression profile of a series of IEGs (c-fos, c-jun, fra-1, Krox-20, Krox-24, arc, sgk-1, BDNF and NARP) in six rat brain regions (prefrontal cortex, hippocampus, striatum, nucleus accumbens, thalamus and cerebellum). Rats (n _ 5) were administered either clozapine (20mg/kg i.p.), haloperidol (1mg/kg i.p.) or the appropriate vehicle with pre-treatment times of 1, 6 and 24 h. IEG expression was analysed in these regions by Taqman RT-PCR. The spatial and temporal profile of IEG induction following anti-psychotic drug treatment correlates with regions associated with the efficacy and side effect profile of each drug. In particular, sgk-1 expression levels after anti-psychotic drug treatment may have predictive value when investigating the profile of a novel anti-psychotic drug.

Key Words: anti-psychotic, immediate early genes, schizophrenia, RT-PCR, clozapine, haloperidol

First published on January 21, 2008, doi:10.1177/0269881107081521

Journal of Psychopharmacology 2008;22:536.

A more recent version of this article appeared on July 1, 2008


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?