This Article Full Text (PDF) All Versions of this Article: 0269881108091075v1 0269881108091075v2 23/5/520    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Scopus Google Scholar Articles by Pennington, K Articles by Cotter, D. PubMed PubMed Citation Articles by Pennington, K Articles by Cotter, D. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Antidepressants Social Bookmarking                         What's this?

A proteomic investigation of similarities between conventional and herbal antidepressant treatments

Department of Psychiatry, Royal College of Surgeons in Ireland, Dublin, Ireland; Proteome Research Centre, UCD Conway Institute, University College Dublin, Dublin, Ireland k.pennington{at}leeds.ac.uk

M Föcking

Department of Psychiatry, Royal College of Surgeons in Ireland, Dublin, Ireland

CA McManus

Proteome Research Centre, UCD Conway Institute, University College Dublin, Dublin, Ireland

CM Pariante

Section of Stress, Psychiatry and Immunology, Division of Psychological Medicine, Institute of Psychiatry, King’s College, London, UK

MJ Dunn

Proteome Research Centre, UCD Conway Institute, University College Dublin, Dublin, Ireland

DR Cotter

Department of Psychiatry, Royal College of Surgeons in Ireland, Dublin, Ireland

Abstract

Increasing clinical evidence for the effectiveness of herbal antidepressants has led to investigations at the molecular level. Using two-dimensional gel electrophoresis, this study investigated similarities in protein expression between clomipramine, St John’s wort and a Chinese herbal formula, xiao-yao-san, often used in mood disorder treatment. HT22 cells, derived from a mouse hippocampal cell line, were treated for 24 h, and protein expression was compared with that of the untreated cells (n = 4/group). Forty-three protein spots were found to be significantly differentially expressed (P < 0.05) in more than one of the treatment groups. Twenty-nine of these were identified using mass spectrometry. The most affected proteins were those involved in the cytoskeleton and energy metabolism, and an up-regulation of vimentin by all three treatments was confirmed by Western blotting. This study provides preliminary evidence for multiple common molecular targets between conventional and alternative antidepressants, which appear to collectively affect neuronal plasticity.

Key Words: 2-DE • antidepressants • Chinese herbal medicine • clomipramine • neuronal plasticity • St John’s wort

This version was published on July 1, 2009

Journal of Psychopharmacology, Vol. 23, No. 5, 520-530 (2009)
DOI: 10.1177/0269881108091075


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?